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rna interference trim28 sirna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rna interference trim28 sirna
    Rna Interference Trim28 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+interference/TR%CE%B22+siRNA/pm41578369-124-0-8
    Average 85 stars, based on 2 article reviews
    rna interference trim28 sirna - by Bioz Stars, 2026-11
    85/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Hypoxia induces oncogene yes-associated protein 1 nuclear translocation to promote pancreatic ductal adenocarcinoma invasion via epithelial-mesenchymal transition.
    Article Snippet: Gene expression vectors and transfection The YAP1 short hairpin RNA (shRNA; 5′-CCCAGTT AAATGTTCACCAAT-3′) and scrambled control shRNA (GenePharma, China) were transfected into PANC-1 and BxPC-3 cells. .. The HIF-1α expression was suppressed by shRNA interference (Santa Cruz Biotechnology), and plasmid-A shRNA (Santa Cruz Biotechnology) was used as a negative control. .. The cells were selected by 1 mg/mL of G418 or 1 μg/mL of puromycin for 2 weeks to form stable clones after transfection in vitro with PolyJetTM DNA transfection reagent (SignaGen Laboratories, USA) according to the manufacturer’s protocol.

    shRNA:

    Article Title: Hypoxia induces oncogene yes-associated protein 1 nuclear translocation to promote pancreatic ductal adenocarcinoma invasion via epithelial-mesenchymal transition.
    Article Snippet: Gene expression vectors and transfection The YAP1 short hairpin RNA (shRNA; 5′-CCCAGTT AAATGTTCACCAAT-3′) and scrambled control shRNA (GenePharma, China) were transfected into PANC-1 and BxPC-3 cells. .. The HIF-1α expression was suppressed by shRNA interference (Santa Cruz Biotechnology), and plasmid-A shRNA (Santa Cruz Biotechnology) was used as a negative control. .. The cells were selected by 1 mg/mL of G418 or 1 μg/mL of puromycin for 2 weeks to form stable clones after transfection in vitro with PolyJetTM DNA transfection reagent (SignaGen Laboratories, USA) according to the manufacturer’s protocol.

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 μg/ml). .. Nuclei and cytosol were isolated using a nuclear extract kit according to the manufacturer's instructions (Active Motif).

    Article Title: Activation of the unfolded protein response contributed to the selective cytotoxicity of oroxylin A in human hepatocellular carcinoma HepG2 cells.
    Article Snippet: Oroxylin A preferentially killed malignant but not normal hepatocytes.. Oroxylin A specifically increased the production of H2O2 in malignant hepatocytes.. H2O2 triggered the unfolded protein response (UPR).

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 g/ml). ..

    Plasmid Preparation:

    Article Title: Hypoxia induces oncogene yes-associated protein 1 nuclear translocation to promote pancreatic ductal adenocarcinoma invasion via epithelial-mesenchymal transition.
    Article Snippet: Gene expression vectors and transfection The YAP1 short hairpin RNA (shRNA; 5′-CCCAGTT AAATGTTCACCAAT-3′) and scrambled control shRNA (GenePharma, China) were transfected into PANC-1 and BxPC-3 cells. .. The HIF-1α expression was suppressed by shRNA interference (Santa Cruz Biotechnology), and plasmid-A shRNA (Santa Cruz Biotechnology) was used as a negative control. .. The cells were selected by 1 mg/mL of G418 or 1 μg/mL of puromycin for 2 weeks to form stable clones after transfection in vitro with PolyJetTM DNA transfection reagent (SignaGen Laboratories, USA) according to the manufacturer’s protocol.

    Article Title: Activation of the unfolded protein response contributed to the selective cytotoxicity of oroxylin A in human hepatocellular carcinoma HepG2 cells.
    Article Snippet: Oroxylin A preferentially killed malignant but not normal hepatocytes.. Oroxylin A specifically increased the production of H2O2 in malignant hepatocytes.. H2O2 triggered the unfolded protein response (UPR).

    Negative Control:

    Article Title: Hypoxia induces oncogene yes-associated protein 1 nuclear translocation to promote pancreatic ductal adenocarcinoma invasion via epithelial-mesenchymal transition.
    Article Snippet: Gene expression vectors and transfection The YAP1 short hairpin RNA (shRNA; 5′-CCCAGTT AAATGTTCACCAAT-3′) and scrambled control shRNA (GenePharma, China) were transfected into PANC-1 and BxPC-3 cells. .. The HIF-1α expression was suppressed by shRNA interference (Santa Cruz Biotechnology), and plasmid-A shRNA (Santa Cruz Biotechnology) was used as a negative control. .. The cells were selected by 1 mg/mL of G418 or 1 μg/mL of puromycin for 2 weeks to form stable clones after transfection in vitro with PolyJetTM DNA transfection reagent (SignaGen Laboratories, USA) according to the manufacturer’s protocol.

    Control:

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 μg/ml). .. Nuclei and cytosol were isolated using a nuclear extract kit according to the manufacturer's instructions (Active Motif).

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 g/ml). ..

    Transfection:

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 μg/ml). .. Nuclei and cytosol were isolated using a nuclear extract kit according to the manufacturer's instructions (Active Motif).

    Article Title: Activation of the unfolded protein response contributed to the selective cytotoxicity of oroxylin A in human hepatocellular carcinoma HepG2 cells.
    Article Snippet: Oroxylin A preferentially killed malignant but not normal hepatocytes.. Oroxylin A specifically increased the production of H2O2 in malignant hepatocytes.. H2O2 triggered the unfolded protein response (UPR).

    Article Title: AMP-activated Protein Kinase Regulates β-Catenin Transcription via Histone Deacetylase 5
    Article Snippet: .. For shRNA interference, HDAC5 shRNA and control shRNA (Santa Cruz Biotechnology, Santa Cruz, CA) were delivered into cells, and transfected cells were selected using puromycin (2 g/ml). ..

    Knockdown:

    Article Title: Activation of the unfolded protein response contributed to the selective cytotoxicity of oroxylin A in human hepatocellular carcinoma HepG2 cells.
    Article Snippet: Oroxylin A preferentially killed malignant but not normal hepatocytes.. Oroxylin A specifically increased the production of H2O2 in malignant hepatocytes.. H2O2 triggered the unfolded protein response (UPR).



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    E2F2 and MYBL2 regulated rumen epithelial cell cycle and apoptosis. A E2F2 silencing efficiency assay. B The expression of genes related to cell cycle and apoptosis transfected with <t>siRNA-</t> E2F2 . C Detection of rumen epithelial cell viability transfected with siRNA- E2F2 . D – E Detection of rumen epithelial cell apoptosis rate transfected with siRNA- E2F2 . F – G Cell cycle detection of rumen epithelial cells transfected with siRNA- E2F2 . H MYBL2 silencing efficiency assay. I The expression of genes related to cell cycle and apoptosis transfected with siRNA- MYBL2 . J Detection of rumen epithelial cell viability transfected with siRNA- MYBL2 . K – L Detection of rumen epithelial cell apoptosis rate transfected with siRNA- MYBL2 . M – N Cell cycle detection of rumen epithelial cells transfected with siRNA- MYBL2. The data were analyzed using two-tailed t -test for comparisons between two groups ( n = 3) or one-way ANOVA for comparisons among three groups ( n = 3), * P < 0.05
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    Galectin Therapeutics sirna interference
    E2F2 and MYBL2 regulated rumen epithelial cell cycle and apoptosis. A E2F2 silencing efficiency assay. B The expression of genes related to cell cycle and apoptosis transfected with <t>siRNA-</t> E2F2 . C Detection of rumen epithelial cell viability transfected with siRNA- E2F2 . D – E Detection of rumen epithelial cell apoptosis rate transfected with siRNA- E2F2 . F – G Cell cycle detection of rumen epithelial cells transfected with siRNA- E2F2 . H MYBL2 silencing efficiency assay. I The expression of genes related to cell cycle and apoptosis transfected with siRNA- MYBL2 . J Detection of rumen epithelial cell viability transfected with siRNA- MYBL2 . K – L Detection of rumen epithelial cell apoptosis rate transfected with siRNA- MYBL2 . M – N Cell cycle detection of rumen epithelial cells transfected with siRNA- MYBL2. The data were analyzed using two-tailed t -test for comparisons between two groups ( n = 3) or one-way ANOVA for comparisons among three groups ( n = 3), * P < 0.05
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    Image Search Results


    Proposed mechanism for CALAA-01. (a) Nanoparticles formed through the assembly of a linear polymer containing cyclodextrin (CDP), an adamantane-PEG conjugate (AD-PEG), a targeting element (transferrin, Tf), and the therapeutic gene (siRNA). (b) Patients receive an infusion of these nanoparticles. (c) Nanoparticles circulate and infiltrate tumors. (d) Receptor-mediated endocytosis occurs. (e) Targeted nanoparticles interact with receptors on the cancer cell surface. Reprinted from with permission from American Chemical Society.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Advances in nanomaterial-mediated CRISPR/Cas delivery: from lipid nanoparticles to vesicle-derived systems

    doi: 10.3389/fbioe.2025.1669104

    Figure Lengend Snippet: Proposed mechanism for CALAA-01. (a) Nanoparticles formed through the assembly of a linear polymer containing cyclodextrin (CDP), an adamantane-PEG conjugate (AD-PEG), a targeting element (transferrin, Tf), and the therapeutic gene (siRNA). (b) Patients receive an infusion of these nanoparticles. (c) Nanoparticles circulate and infiltrate tumors. (d) Receptor-mediated endocytosis occurs. (e) Targeted nanoparticles interact with receptors on the cancer cell surface. Reprinted from with permission from American Chemical Society.

    Article Snippet: Hereditary transthyretin amyloidosis (hATTR) , RNA interference (siRNA) , Lipid nanoparticles (LNPs) , Reduces transthyretin protein production , Alnylam Pharmaceuticals , .

    Techniques: Polymer

    E2F2 and MYBL2 regulated rumen epithelial cell cycle and apoptosis. A E2F2 silencing efficiency assay. B The expression of genes related to cell cycle and apoptosis transfected with siRNA- E2F2 . C Detection of rumen epithelial cell viability transfected with siRNA- E2F2 . D – E Detection of rumen epithelial cell apoptosis rate transfected with siRNA- E2F2 . F – G Cell cycle detection of rumen epithelial cells transfected with siRNA- E2F2 . H MYBL2 silencing efficiency assay. I The expression of genes related to cell cycle and apoptosis transfected with siRNA- MYBL2 . J Detection of rumen epithelial cell viability transfected with siRNA- MYBL2 . K – L Detection of rumen epithelial cell apoptosis rate transfected with siRNA- MYBL2 . M – N Cell cycle detection of rumen epithelial cells transfected with siRNA- MYBL2. The data were analyzed using two-tailed t -test for comparisons between two groups ( n = 3) or one-way ANOVA for comparisons among three groups ( n = 3), * P < 0.05

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Maternal undernutrition inhibits fetal rumen development: novel miRNA-736-mediated dual targeting of E2F2 and MYBL2 in sheep

    doi: 10.1186/s40104-025-01321-7

    Figure Lengend Snippet: E2F2 and MYBL2 regulated rumen epithelial cell cycle and apoptosis. A E2F2 silencing efficiency assay. B The expression of genes related to cell cycle and apoptosis transfected with siRNA- E2F2 . C Detection of rumen epithelial cell viability transfected with siRNA- E2F2 . D – E Detection of rumen epithelial cell apoptosis rate transfected with siRNA- E2F2 . F – G Cell cycle detection of rumen epithelial cells transfected with siRNA- E2F2 . H MYBL2 silencing efficiency assay. I The expression of genes related to cell cycle and apoptosis transfected with siRNA- MYBL2 . J Detection of rumen epithelial cell viability transfected with siRNA- MYBL2 . K – L Detection of rumen epithelial cell apoptosis rate transfected with siRNA- MYBL2 . M – N Cell cycle detection of rumen epithelial cells transfected with siRNA- MYBL2. The data were analyzed using two-tailed t -test for comparisons between two groups ( n = 3) or one-way ANOVA for comparisons among three groups ( n = 3), * P < 0.05

    Article Snippet: The small interference RNA (siRNA) for E2F2 and MYBL2 were synthesized by Sangon Biotech (Shanghai, China).

    Techniques: Expressing, Transfection, Two Tailed Test